Hotspot

ycf1

SSC · 130048-135636 · 5589 bp

Back to Heteranthera

Pi 0.0277

MarkerSeek score 65.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5589
variable_sites: 599
pi: 0.0277
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTACGTCTTTGAGCACGCAT	TCGTGCAAGAAAAGCCAAAC	45.0	45.0	58.2	57.8	210-240 bp	0.909091	85.1
ycf1_p2	TTTCGGGGAGAACCAAAAGG	TCGTGCAAGAAAAGCCAAAC	50.0	45.0	58.0	57.8	864-894 bp	0.909091	84.2
ycf1_p3	AGGTTGTTTTCGGGGAGAAC	TCGTGCAAGAAAAGCCAAAC	50.0	45.0	58.0	57.8	871-901 bp	0.909091	84.1
ycf1_p4	GAAGGTTGTTTTCGGGGAGA	TCGTGCAAGAAAAGCCAAAC	50.0	45.0	57.7	57.8	873-903 bp	0.909091	82.2
ycf1_p5	AATGCTATCCCAAGCTTCCG	TCGTGCAAGAAAAGCCAAAC	50.0	45.0	58.0	57.8	466-496 bp	0.636364	70.3