Hotspot

psaC-ndhE

SSC · 123196-123834 · 639 bp

Back to Heteranthera

Pi 0.0383

MarkerSeek score 81.80

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 639
variable_sites: 75
pi: 0.0383
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TCGTACACATTGAGTACACCC	CCGGAACAGAAAATCAACTCG	47.6	47.6	57.4	57.5	722-762 bp	1.0	67.7
psaC-ndhE_p2	CGTACACATTGAGTACACCCT	CCGGAACAGAAAATCAACTCG	47.6	47.6	57.4	57.5	721-761 bp	1.0	67.7
psaC-ndhE_p3	CGTACACATTGAGTACACCC	CCGGAACAGAAAATCAACTCG	50.0	47.6	55.9	57.5	721-761 bp	1.0	63.5
psaC-ndhE_p4	GCTCGTACACATTGAGTACAC	CCGGAACAGAAAATCAACTCG	47.6	47.6	56.8	57.5	724-764 bp	1.0	62.4
psaC-ndhE_p5	TCGTACACATTGAGTACACCC	CACCGGAACAGAAAATCAACTC	47.6	45.5	57.4	57.9	724-764 bp	1.0	62.2