Hotspot

rpl32-trnL-UAG

SSC · 119350-119949 · 600 bp

Back to Heteranthera

Pi 0.05

MarkerSeek score 87.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 600
variable_sites: 107
pi: 0.05
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTTTTGTGCGACAAACAAAT	TCCTAAGAGCAGCGTGTCTA	30.0	50.0	53.4	57.9	671-695 bp	1.0	53.3
rpl32-trnL-UAG_p2	TTTTTGTGCGACAAACAAAT	AGCAGCGTGTCTACCAATTT	30.0	45.0	53.4	57.8	664-688 bp	1.0	52.9
rpl32-trnL-UAG_p3	TTTTTGTGCGACAAACAAAT	CTAAGAGCAGCGTGTCTACC	30.0	55.0	53.4	57.8	669-693 bp	1.0	52.8
rpl32-trnL-UAG_p4	TTTTTGTGCGACAAACAAAT	CCTAAGAGCAGCGTGTCTAC	30.0	55.0	53.4	57.8	670-694 bp	1.0	52.7
rpl32-trnL-UAG_p5	TTTTTGTGCGACAAACAAAT	ACAGTATAAGATGCCGCCAC	30.0	50.0	53.4	57.8	723-747 bp	1.0	52.2