Hotspot

petD-rpoA

LSC · 82948-83144 · 197 bp

Back to Heteranthera

Pi 0.0554

MarkerSeek score 77.70

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 34
pi: 0.0554
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TTGATTGGTACCGCAGTAGC	TGCTGTATCCATGCCTGTTC	50.0	50.0	58.0	58.0	779-792 bp	1.0	87.2
petD-rpoA_p2	TAATGGTTTCAGTACCGGCG	TGCTGTATCCATGCCTGTTC	50.0	50.0	58.0	58.0	880-893 bp	1.0	86.6
petD-rpoA_p3	TAATGGTTTCAGTACCGGCG	AGAGATGCATCCATTTGCGT	50.0	45.0	58.0	58.0	1089-1102 bp	1.0	85.6
petD-rpoA_p4	TTGATTGGTACCGCAGTAGC	ACCGTACGAAACCCACAAAA	50.0	45.0	58.0	58.0	828-841 bp	0.727273	74.6
petD-rpoA_p5	TAATGGTTTCAGTACCGGCG	ACCGTACGAAACCCACAAAA	50.0	45.0	58.0	58.0	929-942 bp	0.727273	74.3