Hotspot

petA-psbJ

LSC · 67788-68914 · 1127 bp

Back to Heteranthera

Pi 0.0327

MarkerSeek score 81.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1127
variable_sites: 135
pi: 0.0327
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTTGGCGCAAGTCTTTTTGG	GGTTCATATTCCGGGTTGGG	45.0	55.0	58.1	58.3	962-1349 bp	1.0	89.0
petA-psbJ_p2	TTTGGCGCAAGTCTTTTTGG	CATATTCCGGGTTGGGTTCA	45.0	50.0	58.1	57.6	958-1345 bp	1.0	88.0
petA-psbJ_p3	TTTGGCGCAAGTCTTTTTGG	GTTCATATTCCGGGTTGGGT	45.0	50.0	58.1	57.6	961-1348 bp	1.0	88.0
petA-psbJ_p4	GCATCTGTTATTTTGGCGCA	GGTTCATATTCCGGGTTGGG	45.0	55.0	57.7	58.3	973-1360 bp	1.0	87.3
petA-psbJ_p5	GCATCTGTTATTTTGGCGCA	CATATTCCGGGTTGGGTTCA	45.0	50.0	57.7	57.6	969-1356 bp	1.0	86.4