Hotspot

psaI-ycf4

LSC · 64357-64742 · 386 bp

Back to Heteranthera

Pi 0.0469

MarkerSeek score 82.30

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 64
pi: 0.0469
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GCAATTGCAATGGCTTCTTT	ATCTTCTGATCGCCAGTTCA	40.0	45.0	56.1	56.6	454-463 bp	1.0	64.8
psaI-ycf4_p2	GCAATTGCAATGGCTTCTTT	TCTTCTGATCGCCAGTTCAT	40.0	45.0	56.1	56.6	453-462 bp	1.0	64.8
psaI-ycf4_p3	GCAATTGCAATGGCTTCTTT	ATCTTCTGATCGCCAGTTCAT	40.0	42.9	56.1	57.2	454-463 bp	1.0	60.9
psaI-ycf4_p4	GCAATTGCAATGGCTTCTTT	TCGAGACCCCGTTATAAGTTC	40.0	47.6	56.1	56.9	484-493 bp	1.0	58.9
psaI-ycf4_p5	GCAATTGCAATGGCTTCTTT	CGAGACCCCGTTATAAGTTCT	40.0	47.6	56.1	56.9	483-492 bp	1.0	58.9