Hotspot

accD-psaI

LSC · 62938-64245 · 1308 bp

Back to Heteranthera

Pi 0.0319

MarkerSeek score 75.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1308
variable_sites: 150
pi: 0.0319
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGGGATCTGTAGTAGGCGAG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1608-1947 bp	1.0	91.5
accD-psaI_p2	TTTTAAAGGGCGTTCTGGCT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	1379-1551 bp	0.818182	84.4
accD-psaI_p3	TGGGATCTGTAGTAGGCGAG	TTGGTTCCACAGAGCCTAGA	55.0	50.0	58.0	58.0	1895-1913 bp	0.272727	52.1
accD-psaI_p4	GTATGTGCTTCTGGAGGAGC	TTGGTTCCACAGAGCCTAGA	55.0	50.0	58.1	58.0	1821-1839 bp	0.272727	51.8
accD-psaI_p5	TTTTAAAGGGCGTTCTGGCT	TTGGTTCCACAGAGCCTAGA	45.0	50.0	58.0	58.0	1499-1507 bp	0.181818	48.1