Hotspot

trnF-GAA-ndhJ

LSC · 51100-51795 · 696 bp

Back to Heteranthera

Pi 0.0267

MarkerSeek score 79.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 696
variable_sites: 72
pi: 0.0267
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ATAGGTTGGCCCCTACGTAA	50.0	50.0	58.1	57.8	610-802 bp	1.0	89.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCTGATGCCTGAAAGTTGGA	50.0	50.0	58.1	57.8	629-821 bp	1.0	89.5
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TCCTGATGCCTGAAAGTTGG	50.0	50.0	58.1	57.8	630-822 bp	1.0	89.5
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GATAGGTTGGCCCCTACGTA	50.0	55.0	58.1	58.3	611-803 bp	1.0	89.0
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	AAAGTTGGATAGGTTGGCCC	50.0	50.0	58.1	57.8	786-810 bp	0.909091	84.7