Hotspot

trnS-UGA-psbZ

LSC · 38183-38529 · 347 bp

Back to Heteranthera

Pi 0.0279

MarkerSeek score 78.20

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 347
variable_sites: 34
pi: 0.0279
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	419-525 bp	1.0	88.2
trnS-UGA-psbZ_p2	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	55.0	55.0	58.0	58.3	421-527 bp	1.0	87.8
trnS-UGA-psbZ_p3	GTTTTCAAGACCGGGGCTAT	CCAACCATCAGGAGAAGCAA	50.0	50.0	57.9	57.8	439-545 bp	1.0	87.1
trnS-UGA-psbZ_p4	GTTTTCAAGACCGGGGCTAT	GACCAACCATCAGGAGAAGC	50.0	55.0	57.9	58.3	441-547 bp	1.0	86.7
trnS-UGA-psbZ_p5	GCTATCAACCACTCAGCCAT	CCAACCATCAGGAGAAGCAA	50.0	50.0	57.7	57.8	424-530 bp	1.0	85.6