Hotspot

psbC-trnS-UGA

LSC · 37967-38089 · 123 bp

Back to Heteranthera

Pi 0.0349

MarkerSeek score 78.00

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 123
variable_sites: 15
pi: 0.0349
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	GCTGCAGCAGGTTTTGAAAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.1	58.0	278-310 bp	1.0	88.1
psbC-trnS-UGA_p2	GCTGCAGCAGGTTTTGAAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	58.1	58.2	209-241 bp	1.0	88.0
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	236-268 bp	1.0	87.8
psbC-trnS-UGA_p4	GCTGCAGCAGGTTTTGAAAA	ATAGCCCCGGTCTTGAAAAC	45.0	50.0	58.1	57.9	258-290 bp	1.0	87.6
psbC-trnS-UGA_p5	GCTGCAGCAGGTTTTGAAAA	TTACCTCTGGGAGAGATGGC	45.0	55.0	58.1	58.2	288-320 bp	1.0	87.0