Hotspot

rps16-trnQ-UUG

LSC · 6129-7566 · 1438 bp

Back to Heteranthera

Pi 0.0388
MarkerSeek score 90.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1438
variable_sites
205
pi
0.0388
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
90.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CCACAACCGATCATGTCCTT GAAATCAAAATGGGGCGTGG 50.0 50.0 57.9 58.0 1584-1653 bp 1.0 91.0
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAAATCAAAATGGGGCGTGG 45.0 50.0 58.1 58.0 1563-1632 bp 1.0 90.8
rps16-trnQ-UUG_p3 CCACAACCGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1526-1595 bp 1.0 90.5
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC GAAATCAAAATGGGGCGTGG 50.0 50.0 58.2 58.0 1574-1643 bp 1.0 90.3
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1505-1574 bp 1.0 90.3