Hotspot

ycf1

SSC · 121934-127504 · 5571 bp

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Pi 0.0308

MarkerSeek score 58.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5571
variable_sites: 255
pi: 0.0308
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCATTTGTCTTTTTGACTCA	AGGTCTCGATTAATACCTAAAGA	33.3	34.8	53.6	54.1	169-175 bp	1.0	50.8
ycf1_p2	TTCCATTTGTCTTTTTGACTCA	AGGTCTCGATTAATACCTAAAGA	31.8	34.8	54.4	54.1	170-176 bp	1.0	50.8
ycf1_p3	TCCATTTGTCTTTTTGACTCA	AAGGTCTCGATTAATACCTAAAGA	33.3	33.3	53.6	54.9	170-176 bp	1.0	50.8
ycf1_p4	TCCATTTGTCTTTTTGACTCA	AGGTCTCGATTAATACCTAAAGAA	33.3	33.3	53.6	54.9	169-175 bp	1.0	50.8
ycf1_p5	TTTCCATTTGTCTTTTTGACTCA	AGGTCTCGATTAATACCTAAAGA	30.4	34.8	55.2	54.1	171-177 bp	1.0	50.8