Hotspot

ycf1-trnN-GUU

IRa · 127505-127837 · 333 bp

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Pi 0.0521

MarkerSeek score 72.00

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 333
variable_sites: 25
pi: 0.0521
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	CCTTCTTCCATAACCCGAGC	ATATGTTTGCTCGCGGGAAT	55.0	45.0	58.0	58.0	836-1295 bp	1.0	82.5
ycf1-trnN-GUU_p2	TCCTCGGTTCCTTCTTCCAT	ATATGTTTGCTCGCGGGAAT	50.0	45.0	58.0	58.0	845-1304 bp	1.0	82.4
ycf1-trnN-GUU_p3	CCTTCTTCCATAACCCGAGC	GGCAGAATAGCAGAGCAAGT	55.0	50.0	58.0	58.0	898-1357 bp	1.0	82.3
ycf1-trnN-GUU_p4	TCCTCGGTTCCTTCTTCCAT	GGCAGAATAGCAGAGCAAGT	50.0	50.0	58.0	58.0	907-1366 bp	1.0	82.3
ycf1-trnN-GUU_p5	TGATACCTCCTCCTCGGTTC	ATATGTTTGCTCGCGGGAAT	55.0	45.0	57.9	58.0	855-1314 bp	1.0	82.2