Hotspot

ndhG-ndhI

SSC · 117063-117232 · 170 bp

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Pi 0.049
MarkerSeek score 72.40
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
170
variable_sites
12
pi
0.049
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 TGTATTGGTCCAGGTAAATCCA TCAACAACGATTACCAATTCCT 40.9 36.4 56.8 56.0 216-217 bp 1.0 47.0
ndhG-ndhI_p2 TGTATTGGTCCAGGTAAATCCA TCAACATCAACAACGATTACCA 40.9 36.4 56.8 56.3 223 bp 0.333333 13.3
ndhG-ndhI_p3 TGTATTGGTCCAGGTAAATCCA TCAACATCAACAACGATTACCAA 40.9 34.8 56.8 57.0 223 bp 0.333333 13.3
ndhG-ndhI_p4 TGTATTGGTCCAGGTAAATCCA TTCAACATCAACAACGATTACCA 40.9 34.8 56.8 57.0 224 bp 0.333333 13.3
ndhG-ndhI_p5 TGTATTGGTCCAGGTAAATCCA ACATCAACAACGATTACCAATTCC 40.9 37.5 56.8 58.1 220 bp 0.333333 13.3