Hotspot

ndhF-rpl32

SSC · 111377-111509 · 133 bp

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Pi 0.0702

MarkerSeek score 73.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 133
variable_sites: 14
pi: 0.0702
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACACATTCCCACAAGTTCCC	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.0	58.0	664-1342 bp	1.0	81.6
ndhF-rpl32_p2	CACATTCCCACAAGTTCCCA	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.0	58.0	663-1341 bp	1.0	81.6
ndhF-rpl32_p3	AAGGGGAAATTGTGCGGATT	TCCACCGGACAGTCAAAAAG	45.0	50.0	58.1	58.0	931-1609 bp	1.0	81.0
ndhF-rpl32_p4	AGGGGAAATTGTGCGGATTT	TCCACCGGACAGTCAAAAAG	45.0	50.0	58.1	58.0	930-1608 bp	1.0	81.0
ndhF-rpl32_p5	GGCCGAAAACCACATAGCTA	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.0	58.0	361-1035 bp	0.666667	68.4