Hotspot

rpl16-rps3

LSC · 80382-81822 · 1441 bp

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Pi 0.0321

MarkerSeek score 76.20

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1441
variable_sites: 67
pi: 0.0321
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CAAGTGCCTGAAGAGCGTAT	GCAGGGTTCCCTTACAAACA	50.0	50.0	58.0	58.0	1614-1625 bp	1.0	85.5
rpl16-rps3_p2	TCGCCCTGCTTCTATTTGTC	GCAGGGTTCCCTTACAAACA	50.0	50.0	58.0	58.0	1655-1666 bp	1.0	85.3
rpl16-rps3_p3	CGCCCTGCTTCTATTTGTCT	GCAGGGTTCCCTTACAAACA	50.0	50.0	58.0	58.0	1654-1665 bp	1.0	85.2
rpl16-rps3_p4	CTAGCTGTGATCCAAGCAGG	GCAGGGTTCCCTTACAAACA	55.0	50.0	58.0	58.0	1636-1647 bp	1.0	85.1
rpl16-rps3_p5	CTGTGATCCAAGCAGGTTCA	GCAGGGTTCCCTTACAAACA	50.0	50.0	57.8	58.0	1632-1643 bp	1.0	84.0