Hotspot

clpP-psbB

LSC · 70429-71025 · 597 bp

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Pi 0.038

MarkerSeek score 71.10

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 597
variable_sites: 30
pi: 0.038
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CAGATAGCTGCCAAGAAGCA	50.0	50.0	58.0	58.0	968-1014 bp	1.0	86.7
clpP-psbB_p2	CGCACTATACGTCAACCCAA	AGATAGCTGCCAAGAAGCAC	50.0	50.0	58.0	58.0	967-1013 bp	1.0	86.7
clpP-psbB_p3	CGCACTATACGTCAACCCAA	ACACCCATATTCCAGGACCA	50.0	50.0	58.0	58.0	1135-1181 bp	1.0	86.4
clpP-psbB_p4	TCCTGAATGGGCCCTCTAAA	ACACCCATATTCCAGGACCA	50.0	50.0	58.0	58.0	1844 bp	0.333333	53.0
clpP-psbB_p5	TTCCTGAATGGGCCCTCTAA	ACACCCATATTCCAGGACCA	50.0	50.0	58.0	58.0	1845 bp	0.333333	53.0