primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	TCTTTGTAATTCTGTCATTTATAGACA	AACGGTAGAGTACTCGGCTT	27	20	25.9	50.0	55.2	58.2	0.0	17.1	9.966134	3220	4185	1018-1043	1.000000	44.1
matK-trnK-UUU_p2	matK-trnK-UUU	2	TCTTTGTAATTCTGTCATTTATAGACA	ACGGTAGAGTACTCGGCTTT	27	20	25.9	50.0	55.2	58.2	0.0	17.1	9.966134	3220	4185	1017-1042	1.000000	44.1
matK-trnK-UUU_p3	matK-trnK-UUU	3	CTTTGTAATTCTGTCATTTATAGACA	ACGGTAGAGTACTCGGCTTT	26	20	26.9	50.0	53.9	58.2	0.0	17.1	10.234363	3220	4185	1016-1041	1.000000	44.1
matK-trnK-UUU_p4	matK-trnK-UUU	4	TCTTTGTAATTCTGTCATTTATAGACA	ATCCGGGTTGCTAACTCAAC	27	20	25.9	50.0	55.2	57.9	0.0	0.0	9.907139	3220	4185	1035-1060	1.000000	44.0
matK-trnK-UUU_p5	matK-trnK-UUU	5	CTTTGTAATTCTGTCATTTATAGACA	ATCCGGGTTGCTAACTCAAC	26	20	26.9	50.0	53.9	57.9	0.0	0.0	10.175367	3220	4185	1034-1059	1.000000	44.0
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	TCGATCCCGAAGAGAAGGAA	20	20	50.0	50.0	58.2	57.9	20.3	0.0	0.307743	4223	4858	836-844	1.000000	83.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	TCGATCCCGAAGAGAAGGAA	20	20	50.0	50.0	58.2	57.9	20.3	0.0	0.307743	4223	4858	835-843	1.000000	83.8
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	GCAAAGACAACGAATTCCGG	20	20	50.0	50.0	58.2	58.0	20.3	0.0	0.204673	4223	4858	1249-1260	1.000000	83.7
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	GCAAAGACAACGAATTCCGG	20	20	50.0	50.0	58.2	58.0	20.3	0.0	0.204673	4223	4858	1248-1259	1.000000	83.7
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AGGATCAGTCGCGGTCTTAT	GCAAAGACAACGAATTCCGG	20	20	50.0	50.0	58.3	58.0	6.1	0.0	0.342627	4223	4858	1334-1345	1.000000	82.4
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	57.9	58.0	1.4	0.0	0.060677	7011	7452	799-820	1.000000	86.2
psbK-psbI_p2	psbK-psbI	2	GTTTGGCAAGCTGCTGTAAG	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	57.9	58.0	1.4	0.0	0.062065	7011	7452	800-821	1.000000	86.2
psbK-psbI_p3	psbK-psbI	3	GTTTGGCAAGCTGCTGTAAG	TCTCCGACTCCCCTGATAAC	20	20	50.0	55.0	57.9	57.9	1.4	0.0	0.124928	7011	7452	732-753	1.000000	85.9
psbK-psbI_p4	psbK-psbI	4	GTTTGGCAAGCTGCTGTAAG	CTCCGACTCCCCTGATAACT	20	20	50.0	55.0	57.9	57.9	1.4	0.0	0.130823	7011	7452	731-752	1.000000	85.9
psbK-psbI_p5	psbK-psbI	5	GTTTGGCAAGCTGCTGTAAG	ACAAAAGAAGTACTGGCCCG	20	20	50.0	50.0	57.9	58.1	1.4	0.0	0.163885	7011	7452	1023-1044	1.000000	84.7
petN-psbM_p1	petN-psbM	1	TAGTATGGGGAAGGAGTGGG	AAAACAGTCAGCCAAAATAAT	20	21	55.0	28.6	57.5	52.2	0.0	0.0	7.339198	27850	28402	603-630	1.000000	44.8
petN-psbM_p2	petN-psbM	2	TAGTATGGGGAAGGAGTGGG	AAACAGTCAGCCAAAATAATT	20	21	55.0	28.6	57.5	52.2	0.0	0.0	7.339198	27850	28402	602-629	1.000000	44.8
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	AAAACAGTCAGCCAAAATAAT	20	21	50.0	28.6	58.2	52.2	0.0	0.0	7.001019	27850	28402	660-687	1.000000	44.4
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	AAACAGTCAGCCAAAATAATT	20	21	50.0	28.6	58.2	52.2	0.0	0.0	7.001019	27850	28402	659-686	1.000000	44.4
petN-psbM_p5	petN-psbM	5	TAGTAAGTCTCGCTTGGGCT	AAAACAGTCAGCCAAAATAATT	20	22	50.0	27.3	58.2	53.1	0.0	0.0	7.083008	27850	28402	660-687	1.000000	44.4
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	44841	45842	1007-1052	1.000000	86.3
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	44841	45842	1015-1060	1.000000	85.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	44841	45842	1039-1084	1.000000	85.4
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	44841	45842	1047-1092	1.000000	84.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	44841	45842	1021-1066	1.000000	84.4
psaI-ycf4_p1	psaI-ycf4	1	GGCAATTGCAATGGCTTCTT	AAAGGATAAAGGCCCAGCAA	20	20	45.0	45.0	57.9	57.4	32.4	0.0	0.725093	58308	58690	518-523	1.000000	78.4
psaI-ycf4_p2	psaI-ycf4	2	GGCAATTGCAATGGCTTCTT	AAGGATAAAGGCCCAGCAAA	20	20	45.0	45.0	57.9	57.4	32.4	0.0	0.725093	58308	58690	517-522	1.000000	78.4
psaI-ycf4_p3	psaI-ycf4	3	GGCAATTGCAATGGCTTCTT	AGGATAAAGGCCCAGCAAAA	20	20	45.0	45.0	57.9	57.4	32.4	0.0	0.725093	58308	58690	516-521	1.000000	78.4
psaI-ycf4_p4	psaI-ycf4	4	GGCAATTGCAATGGCTTCTT	AAAAGGATAAAGGCCCAGCA	20	20	45.0	45.0	57.9	57.4	32.4	0.0	0.725093	58308	58690	519-524	1.000000	78.3
psaI-ycf4_p5	psaI-ycf4	5	TAGTCTTTCCGGCAATTGCA	AGGATAAAGGCCCAGCAAAA	20	20	45.0	45.0	57.8	57.4	30.3	0.0	0.814220	58308	58690	526-531	1.000000	77.6
clpP-psbB_p1	clpP-psbB	1	CGCACTATACGTCAACCCAA	CAGATAGCTGCCAAGAAGCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035056	70429	71025	968-1014	1.000000	86.7
clpP-psbB_p2	clpP-psbB	2	CGCACTATACGTCAACCCAA	AGATAGCTGCCAAGAAGCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.036482	70429	71025	967-1013	1.000000	86.7
clpP-psbB_p3	clpP-psbB	3	CGCACTATACGTCAACCCAA	ACACCCATATTCCAGGACCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.021142	70429	71025	1135-1181	1.000000	86.4
clpP-psbB_p4	clpP-psbB	4	TCCTGAATGGGCCCTCTAAA	ACACCCATATTCCAGGACCA	20	20	50.0	50.0	58.0	58.0	25.7	0.0	0.040381	70429	71025	1844-1844	0.333333	53.0
clpP-psbB_p5	clpP-psbB	5	TTCCTGAATGGGCCCTCTAA	ACACCCATATTCCAGGACCA	20	20	50.0	50.0	58.0	58.0	25.7	0.0	0.040381	70429	71025	1845-1845	0.333333	53.0
rpl16-rps3_p1	rpl16-rps3	1	CAAGTGCCTGAAGAGCGTAT	GCAGGGTTCCCTTACAAACA	20	20	50.0	50.0	58.0	58.0	0.0	7.8	0.026030	80382	81822	1614-1625	1.000000	85.5
rpl16-rps3_p2	rpl16-rps3	2	TCGCCCTGCTTCTATTTGTC	GCAGGGTTCCCTTACAAACA	20	20	50.0	50.0	58.0	58.0	0.0	7.8	0.034469	80382	81822	1655-1666	1.000000	85.3
rpl16-rps3_p3	rpl16-rps3	3	CGCCCTGCTTCTATTTGTCT	GCAGGGTTCCCTTACAAACA	20	20	50.0	50.0	58.0	58.0	0.0	7.8	0.040132	80382	81822	1654-1665	1.000000	85.2
rpl16-rps3_p4	rpl16-rps3	4	CTAGCTGTGATCCAAGCAGG	GCAGGGTTCCCTTACAAACA	20	20	55.0	50.0	58.0	58.0	16.5	7.8	0.059466	80382	81822	1636-1647	1.000000	85.1
rpl16-rps3_p5	rpl16-rps3	5	CTGTGATCCAAGCAGGTTCA	GCAGGGTTCCCTTACAAACA	20	20	50.0	50.0	57.8	58.0	5.2	7.8	0.199888	80382	81822	1632-1643	1.000000	84.0
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	TTGTTGCACCCTGTGAATCA	20	20	50.0	45.0	58.0	57.9	7.6	0.0	0.073634	85660	85727	311-483	1.000000	80.8
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	AATCATGGTCCTTGCGGATC	20	20	50.0	50.0	58.0	58.0	7.6	0.0	0.036329	85660	85727	797-969	1.000000	80.5
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	CACTTGACTGTCTACGGCTC	AATCATGGTCCTTGCGGATC	20	20	55.0	50.0	58.0	58.0	9.5	0.0	0.043155	85660	85727	1370-1542	1.000000	80.4
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	GTTGGGCGCTTTAACCATTC	CGAACCCCGGTTAGATTCAG	20	20	50.0	55.0	58.0	58.1	7.6	0.0	0.073268	85660	85727	634-806	1.000000	80.4
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	CCACAACGATGCTGTCCATA	AATCATGGTCCTTGCGGATC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.049892	85660	85727	1417-1589	1.000000	80.3
ndhF-rpl32_p1	ndhF-rpl32	1	ACACATTCCCACAAGTTCCC	TCCACCGGACAGTCAAAAAG	20	20	50.0	50.0	58.0	58.0	0.0	9.5	0.042830	111377	111509	664-1342	1.000000	81.6
ndhF-rpl32_p2	ndhF-rpl32	2	CACATTCCCACAAGTTCCCA	TCCACCGGACAGTCAAAAAG	20	20	50.0	50.0	58.0	58.0	0.0	9.5	0.044251	111377	111509	663-1341	1.000000	81.6
ndhF-rpl32_p3	ndhF-rpl32	3	AAGGGGAAATTGTGCGGATT	TCCACCGGACAGTCAAAAAG	20	20	45.0	50.0	58.1	58.0	0.0	9.5	0.101015	111377	111509	931-1609	1.000000	81.0
ndhF-rpl32_p4	ndhF-rpl32	4	AGGGGAAATTGTGCGGATTT	TCCACCGGACAGTCAAAAAG	20	20	45.0	50.0	58.1	58.0	0.0	9.5	0.101015	111377	111509	930-1608	1.000000	81.0
ndhF-rpl32_p5	ndhF-rpl32	5	GGCCGAAAACCACATAGCTA	TCCACCGGACAGTCAAAAAG	20	20	50.0	50.0	58.0	58.0	0.0	9.5	0.070887	111377	111509	361-1035	0.666667	68.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CTTTTTGACTGTCCGGTGGA	GTCCCGAAGAAATCCAACGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.127156	111684	112429	896-1401	1.000000	87.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CTTTTTGACTGTCCGGTGGA	TGGCGAAACCTTGGGTAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.107041	111684	112429	1043-1548	1.000000	87.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGCCGAAAACCACATAGCTA	GTCCCGAAGAAATCCAACGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.121630	111684	112429	1580-2416	0.666667	69.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGCCGAAAACCACATAGCTA	TGGCGAAACCTTGGGTAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.101515	111684	112429	1727-2563	0.666667	69.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGATTGGCCGAAAACCACAT	TGGCGAAACCTTGGGTAAAA	20	20	45.0	45.0	58.1	57.9	0.0	0.0	0.145478	111684	112429	1732-1732	0.333333	52.2
ccsA-ndhD_p1	ccsA-ndhD	1	TTTTACCCAAGGTTTCGCCA	TTTTTGATTCTGGGCCACGA	20	20	45.0	45.0	57.9	58.0	0.0	0.0	0.080541	113583	113856	1059-1087	1.000000	84.4
ccsA-ndhD_p2	ccsA-ndhD	2	TTTTACCCAAGGTTTCGCCA	TTCTCGATGGCTTCTCTTGC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.084570	113583	113856	1279-1307	1.000000	83.9
ccsA-ndhD_p3	ccsA-ndhD	3	TTTTACCCAAGGTTTCGCCA	CTCGATGGCTTCTCTTGCAT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.118006	113583	113856	1277-1305	1.000000	83.7
ccsA-ndhD_p4	ccsA-ndhD	4	GCATGGGGCTCTTATTGGAA	TTTTTGATTCTGGGCCACGA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.083484	113583	113856	647-654	1.000000	83.3
ccsA-ndhD_p5	ccsA-ndhD	5	GCATGGGGCTCTTATTGGAA	TTCTCGATGGCTTCTCTTGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.087513	113583	113856	867-874	1.000000	82.9
ndhG-ndhI_p1	ndhG-ndhI	1	TGTATTGGTCCAGGTAAATCCA	TCAACAACGATTACCAATTCCT	22	22	40.9	36.4	56.8	56.0	0.0	0.0	7.209467	117063	117232	216-217	1.000000	47.0
ndhG-ndhI_p2	ndhG-ndhI	2	TGTATTGGTCCAGGTAAATCCA	TCAACATCAACAACGATTACCA	22	22	40.9	36.4	56.8	56.3	0.0	0.0	6.909261	117063	117232	223-223	0.333333	13.3
ndhG-ndhI_p3	ndhG-ndhI	3	TGTATTGGTCCAGGTAAATCCA	TCAACATCAACAACGATTACCAA	22	23	40.9	34.8	56.8	57.0	0.0	0.0	7.220233	117063	117232	223-223	0.333333	13.3
ndhG-ndhI_p4	ndhG-ndhI	4	TGTATTGGTCCAGGTAAATCCA	TTCAACATCAACAACGATTACCA	22	23	40.9	34.8	56.8	57.0	0.0	0.0	7.220233	117063	117232	224-224	0.333333	13.3
ndhG-ndhI_p5	ndhG-ndhI	5	TGTATTGGTCCAGGTAAATCCA	ACATCAACAACGATTACCAATTCC	22	24	40.9	37.5	56.8	58.1	0.0	0.0	7.259496	117063	117232	220-220	0.333333	13.3
ndhI-ndhA_p1	ndhI-ndhA	1	TCCAATGTACCTTGCAGCTC	ACCAGAAGCAGAGGAGGAAT	20	20	50.0	50.0	57.9	58.0	5.4	0.0	0.149875	117743	117855	576-590	1.000000	81.8
ndhI-ndhA_p2	ndhI-ndhA	2	ACGAAAACGCTCTGATGTGA	ACCAGAAGCAGAGGAGGAAT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.184930	117743	117855	669-683	1.000000	81.3
ndhI-ndhA_p3	ndhI-ndhA	3	ACGCGAACACATACTTCACA	ACCAGAAGCAGAGGAGGAAT	20	20	45.0	50.0	57.9	58.0	15.9	0.0	0.179022	117743	117855	725-739	1.000000	81.1
ndhI-ndhA_p4	ndhI-ndhA	4	TCCAATGTACCTTGCAGCTC	GCCCATAGAGAAGCCGTATG	20	20	50.0	55.0	57.9	57.9	5.4	0.0	0.199915	117743	117855	807-821	1.000000	80.9
ndhI-ndhA_p5	ndhI-ndhA	5	TCCAATGTACCTTGCAGCTC	TAGGCTTCATGTGCGGTTTT	20	20	50.0	45.0	57.9	58.1	5.4	0.0	0.209574	117743	117855	782-796	1.000000	80.8
ycf1_p1	ycf1	1	TCCATTTGTCTTTTTGACTCA	AGGTCTCGATTAATACCTAAAGA	21	23	33.3	34.8	53.6	54.1	0.0	5.9	12.347564	123583	123702	169-175	1.000000	50.8
ycf1_p2	ycf1	2	TTCCATTTGTCTTTTTGACTCA	AGGTCTCGATTAATACCTAAAGA	22	23	31.8	34.8	54.4	54.1	0.0	5.9	12.494985	123583	123702	170-176	1.000000	50.8
ycf1_p3	ycf1	3	TCCATTTGTCTTTTTGACTCA	AAGGTCTCGATTAATACCTAAAGA	21	24	33.3	33.3	53.6	54.9	0.0	7.9	12.580015	123583	123702	170-176	1.000000	50.8
ycf1_p4	ycf1	4	TCCATTTGTCTTTTTGACTCA	AGGTCTCGATTAATACCTAAAGAA	21	24	33.3	33.3	53.6	54.9	0.0	5.9	12.580015	123583	123702	169-175	1.000000	50.8
ycf1_p5	ycf1	5	TTTCCATTTGTCTTTTTGACTCA	AGGTCTCGATTAATACCTAAAGA	23	23	30.4	34.8	55.2	54.1	0.0	5.9	12.716404	123583	123702	171-177	1.000000	50.8
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	CCTTCTTCCATAACCCGAGC	ATATGTTTGCTCGCGGGAAT	20	20	55.0	45.0	58.0	58.0	0.0	12.0	0.077754	127505	127837	836-1295	1.000000	82.5
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	TCCTCGGTTCCTTCTTCCAT	ATATGTTTGCTCGCGGGAAT	20	20	50.0	45.0	58.0	58.0	0.0	12.0	0.080060	127505	127837	845-1304	1.000000	82.4
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	CCTTCTTCCATAACCCGAGC	GGCAGAATAGCAGAGCAAGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.080603	127505	127837	898-1357	1.000000	82.3
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	TCCTCGGTTCCTTCTTCCAT	GGCAGAATAGCAGAGCAAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.082909	127505	127837	907-1366	1.000000	82.3
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	TGATACCTCCTCCTCGGTTC	ATATGTTTGCTCGCGGGAAT	20	20	55.0	45.0	57.9	58.0	0.0	12.0	0.101516	127505	127837	855-1314	1.000000	82.2
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	TTGTTGCACCCTGTGAATCA	GTTGGGCGCTTTAACCATTC	20	20	45.0	50.0	57.9	58.0	0.0	7.6	0.073634	149903	149970	311-483	1.000000	80.8
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	AATCATGGTCCTTGCGGATC	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.036329	149903	149970	797-969	1.000000	80.5
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	AATCATGGTCCTTGCGGATC	CACTTGACTGTCTACGGCTC	20	20	50.0	55.0	58.0	58.0	0.0	9.5	0.043155	149903	149970	1370-1542	1.000000	80.4
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	CGAACCCCGGTTAGATTCAG	GTTGGGCGCTTTAACCATTC	20	20	55.0	50.0	58.1	58.0	0.0	7.6	0.073268	149903	149970	634-806	1.000000	80.4
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	AATCATGGTCCTTGCGGATC	CCACAACGATGCTGTCCATA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.049892	149903	149970	1417-1589	1.000000	80.3