Hotspot

ndhF

SSC · 112611-114830 · 2220 bp

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Pi 0.0108
MarkerSeek score 49.40
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2220
variable_sites
34
pi
0.0108
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 ACAGGAACAAGAGGGATCCA TCCCATCAATCAGTTCCACA 50.0 45.0 58.0 56.4 2896 bp 0.333333 40.4
ndhF_p2 GGATCCACCGAACAAGACAA TCCCATCAATCAGTTCCACA 50.0 45.0 57.8 56.4 2883 bp 0.333333 39.4
ndhF_p3 ACAGGAACAAGAGGGATCCA TCCCATCAATCAGTTCCACAA 50.0 42.9 58.0 57.2 2896 bp 0.333333 38.5
ndhF_p4 CAGGAACAAGAGGGATCCAC TCCCATCAATCAGTTCCACA 55.0 45.0 57.6 56.4 2895 bp 0.333333 37.5
ndhF_p5 GGATCCACCGAACAAGACAA TCCCATCAATCAGTTCCACAA 50.0 42.9 57.8 57.2 2883 bp 0.333333 37.5