Hotspot

rps15-ycf1

SSC · 126207-126580 · 374 bp

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Pi 0.025
MarkerSeek score 57.90
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
374
variable_sites
14
pi
0.025
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 GAAATTCAACAGATCCGCAGT ACATATGTATCCACGGTTCAGA 42.9 40.9 56.9 56.7 475-478 bp 1.0 42.4
rps15-ycf1_p2 TCAACAGATCCGCAGTTTTT ACATATGTATCCACGGTTCAGA 40.0 40.9 55.9 56.7 470-473 bp 1.0 42.4
rps15-ycf1_p3 TTCAACAGATCCGCAGTTTT ACATATGTATCCACGGTTCAGA 40.0 40.9 55.9 56.7 471-474 bp 1.0 42.4
rps15-ycf1_p4 TGAAATTCAACAGATCCGCAG ACATATGTATCCACGGTTCAGA 42.9 40.9 56.9 56.7 476-479 bp 1.0 42.3
rps15-ycf1_p5 TGAAATTCAACAGATCCGCA ACATATGTATCCACGGTTCAGA 40.0 40.9 55.7 56.7 476-479 bp 1.0 42.3