Hotspot

rps3-rpl22

LSC · 85433-85465 · 33 bp

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Pi 0.0404
MarkerSeek score 64.80
Primer available yes
Label rps3-rpl22

Feature Metadata

label_name
rps3-rpl22
feature_type
igs
region
LSC
length_bp
33
variable_sites
2
pi
0.0404
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rpl22_p1 TCGAAGCCCGAGGATATTCT TCTGACTTTCGCGAGACATG 50.0 50.0 58.0 58.0 701-723 bp 1.0 81.2
rps3-rpl22_p2 TCGAAGCCCGAGGATATTCT ATTCATTGGCGGGGGATAAC 50.0 50.0 58.0 58.0 613 bp 0.333333 53.3
rps3-rpl22_p3 TCGAAGCCCGAGGATATTCT CATTCATTGGCGGGGGATAA 50.0 50.0 58.0 58.0 614 bp 0.333333 53.3
rps3-rpl22_p4 TCGAAGCCCGAGGATATTCT AGCGCGAAGAGTAATCGATC 50.0 50.0 58.0 58.0 519 bp 0.333333 53.0
rps3-rpl22_p5 CACTCGACACGTGCAATTTC ATTCATTGGCGGGGGATAAC 50.0 50.0 58.1 58.0 979 bp 0.333333 52.8