Hotspot

petA-psbJ

LSC · 65065-66183 · 1119 bp

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Pi 0.0176
MarkerSeek score 68.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1119
variable_sites
29
pi
0.0176
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CGTCCAAGGCCTTTTGTTTT GGTTCATATTCCGGGTTGGG 45.0 55.0 57.4 58.3 1261-1267 bp 1.0 75.7
petA-psbJ_p2 CGTCCAAGGCCTTTTGTTTT CATATTCCGGGTTGGGTTCA 45.0 50.0 57.4 57.6 1257-1263 bp 1.0 74.8
petA-psbJ_p3 CGTCCAAGGCCTTTTGTTTT GTTCATATTCCGGGTTGGGT 45.0 50.0 57.4 57.6 1260-1266 bp 1.0 74.8
petA-psbJ_p4 GATCCATTACGCGTCCAAGG GGTTCATATTCCGGGTTGGG 55.0 55.0 58.8 58.3 1272-1278 bp 1.0 74.2
petA-psbJ_p5 CGTCCAAGGCCTTTTGTTTT CGGTATTCCTGTGATCGGTT 45.0 50.0 57.4 57.4 1301-1307 bp 1.0 73.3