Hotspot

trnF-GAA-ndhJ

LSC · 49353-50046 · 694 bp

Back to Hessea

Pi 0.00677
MarkerSeek score 63.70
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
694
variable_sites
7
pi
0.00677
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGAAAGTTGGATAGGCTGGC 50.0 50.0 58.1 57.9 804 bp 1.0 79.7
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA ATAGGCTGGCCCTTACGTAA 50.0 50.0 58.1 58.2 794 bp 1.0 79.2
trnF-GAA-ndhJ_p3 AATCGTCGGGATAGCTCAGT TGAAAGTTGGATAGGCTGGC 50.0 50.0 58.3 57.9 847 bp 1.0 77.7
trnF-GAA-ndhJ_p4 ATCGTCGGGATAGCTCAGTT TGAAAGTTGGATAGGCTGGC 50.0 50.0 58.3 57.9 846 bp 1.0 77.7
trnF-GAA-ndhJ_p5 GAATCGTCGGGATAGCTCAG TGAAAGTTGGATAGGCTGGC 55.0 50.0 57.7 57.9 848 bp 1.0 77.4