Hotspot

psbC-trnS-UGA

LSC · 36628-36746 · 119 bp

Back to Hessea

Pi 0.0504
MarkerSeek score 62.10
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
119
variable_sites
9
pi
0.0504
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CCTGTTCTTTCCATGACCCC GGTGGCTGAGTGGTTGATAG 55.0 55.0 58.2 58.0 232 bp 1.0 81.6
psbC-trnS-UGA_p2 CCTGTTCTTTCCATGACCCC GGTTGATAGCTCCGGTCTTG 55.0 55.0 58.2 58.1 221 bp 1.0 81.5
psbC-trnS-UGA_p3 CCTGTTCTTTCCATGACCCC GCTGAGTGGTTGATAGCTCC 55.0 55.0 58.2 58.1 228 bp 1.0 81.4
psbC-trnS-UGA_p4 CCTGTTCTTTCCATGACCCC GGCTGAGTGGTTGATAGCTC 55.0 55.0 58.2 58.1 229 bp 1.0 81.4
psbC-trnS-UGA_p5 TTCCATGACCCCTCTTAGCT GGTGGCTGAGTGGTTGATAG 50.0 55.0 57.7 58.0 224 bp 1.0 80.8