Hotspot

petA-psbJ

LSC · 66553-67562 · 1010 bp

Back to Hesperomeles

Pi 0.00513

MarkerSeek score 59.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1010
variable_sites: 8
pi: 0.00513
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGCTCTTCTTGGCATCTGTT	AGCCGGTATTCTTGTGATCG	45.0	50.0	57.4	57.8	1151-1182 bp	1.0	76.8
petA-psbJ_p2	TTGCTCTTCTTGGCATCTGT	AGCCGGTATTCTTGTGATCG	45.0	50.0	57.4	57.8	1152-1183 bp	1.0	76.8
petA-psbJ_p3	CGTGTCCAAGGTCTTTTGCT	AGCCGGTATTCTTGTGATCG	50.0	50.0	58.7	57.8	1167-1198 bp	1.0	75.8
petA-psbJ_p4	GGTCTTTTGCTCTTCTTGGC	AGCCGGTATTCTTGTGATCG	50.0	50.0	57.3	57.8	1158-1189 bp	1.0	75.6
petA-psbJ_p5	TCCATTACGTGTCCAAGGTC	AGCCGGTATTCTTGTGATCG	50.0	50.0	57.2	57.8	1174-1205 bp	1.0	75.3