Hotspot

accD-psaI

LSC · 62270-63082 · 813 bp

Back to Hesperomeles

Pi 0.00314

MarkerSeek score 62.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 813
variable_sites: 7
pi: 0.00314
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGGGCGTTCTGAGTGAGTTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.4	58.0	938-959 bp	1.0	80.3
accD-psaI_p2	AGGGCGTTCTGAGTGAGTTA	AATGGAGGGGAGACTGTTGA	50.0	50.0	58.4	58.0	895-916 bp	1.0	80.3
accD-psaI_p3	AGGGCGTTCTGAGTGAGTTA	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.4	58.0	929-950 bp	1.0	80.1
accD-psaI_p4	AGGGCGTTCTGAGTGAGTTA	GAGGGGAGACTGTTGAGAGT	50.0	55.0	58.4	58.1	891-912 bp	1.0	80.1
accD-psaI_p5	AGGGCGTTCTGAGTGAGTTA	GCACAAAAATGGAGGGGAGA	50.0	50.0	58.4	58.1	902-923 bp	1.0	80.0