Hotspot

psbZ-trnG-UCC

LSC · 38936-39381 · 446 bp

Back to Hesperomeles

Pi 0.00908

MarkerSeek score 61.60

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 8
pi: 0.00908
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	583-650 bp	1.0	81.5
psbZ-trnG-UCC_p2	GGGTTGGTCGGGTAACAAAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	569-636 bp	1.0	81.2
psbZ-trnG-UCC_p3	TTCCGGCACATCATTATGGA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.0	57.8	539-606 bp	1.0	75.6
psbZ-trnG-UCC_p4	AGGGTTGGTCGGGTAACAAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	59.2	57.8	570-637 bp	1.0	74.5
psbZ-trnG-UCC_p5	ATTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.2	57.8	584-651 bp	1.0	74.1