Hotspot

trnD-GUC-trnY-GUA

LSC · 32833-33291 · 459 bp

Back to Hesperomeles

Pi 0.0061

MarkerSeek score 63.30

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 459
variable_sites: 6
pi: 0.0061
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	ATTTGGATTCGTCGGGACTG	TGGCAATATGTCTACGCTGG	50.0	50.0	58.0	57.8	570-585 bp	1.0	80.8
trnD-GUC-trnY-GUA_p2	ATTTGGATTCGTCGGGACTG	GGCAATATGTCTACGCTGGT	50.0	50.0	58.0	57.8	569-584 bp	1.0	80.8
trnD-GUC-trnY-GUA_p3	ATTTGGATTCGTCGGGACTG	GGGGACGGACTGTAAATTCG	50.0	55.0	58.0	58.4	591-606 bp	1.0	79.8
trnD-GUC-trnY-GUA_p4	ATTTGGATTCGTCGGGACTG	GGGACGGACTGTAAATTCGT	50.0	50.0	58.0	57.6	590-605 bp	1.0	79.6
trnD-GUC-trnY-GUA_p5	ATTTGGATTCGTCGGGACTG	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.0	58.4	624-639 bp	1.0	79.2