Hotspot

trnR-UCU-atpA

LSC · 10022-10885 · 864 bp

Back to Hesperomeles

Pi 0.0431

MarkerSeek score 82.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 864
variable_sites: 65
pi: 0.0431
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGAATGAAAAGCGTCCATTG	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	851-1062 bp	1.0	73.8
trnR-UCU-atpA_p2	GGAATGAAAAGCGTCCATTGT	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	850-1061 bp	1.0	73.8
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AGACATTTACCGACGAAGCG	47.6	50.0	57.3	58.4	790-1001 bp	1.0	73.2
trnR-UCU-atpA_p4	TGGATAGGACAGAGGTCTTCT	AGACATTTACCGACGAAGCG	47.6	50.0	56.9	58.4	825-1036 bp	1.0	70.1
trnR-UCU-atpA_p5	TGGAATGAAAAGCGTCCATT	AGACATTTACCGACGAAGCG	40.0	50.0	55.9	58.4	851-1062 bp	1.0	69.8