Hotspot

trnK-UUU-rps16

LSC · 4213-5185 · 973 bp

Back to Hesperomeles

Pi 0.00363
MarkerSeek score 48.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
973
variable_sites
10
pi
0.00363
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG CGACGAGAGGGAAGAGATCT 55.0 55.0 58.0 58.0 1278-1313 bp 1.0 81.8
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1380-1416 bp 1.0 81.5
trnK-UUU-rps16_p3 TCAGGATCAGTCGTGGTCTT CGACGAGAGGGAAGAGATCT 50.0 55.0 58.1 58.0 1274-1309 bp 1.0 81.4
trnK-UUU-rps16_p4 TTCAGGATCAGTCGTGGTCT CGACGAGAGGGAAGAGATCT 50.0 55.0 58.1 58.0 1275-1310 bp 1.0 81.4
trnK-UUU-rps16_p5 AACCGTAGTAGGGCTCTACC CGACGAGAGGGAAGAGATCT 55.0 55.0 57.9 58.0 1441-1476 bp 1.0 81.2