Hotspot

rps19

Genome · 15760-16125 · 366 bp

Back to Hesperocyparis

Pi 0.00956

MarkerSeek score 51.20

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: Genome
length_bp: 366
variable_sites: 7
pi: 0.00956
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TGTTTCAATTCTTCGTCGACG	TCAATTTCATCTTCATCCTCCA	42.9	36.4	57.2	55.1	512-519 bp	1.0	41.1
rps19_p2	GTTTCAATTCTTCGTCGACGT	TCAATTTCATCTTCATCCTCCA	42.9	36.4	57.2	55.1	511-518 bp	1.0	41.1
rps19_p3	TGTTTCAATTCTTCGTCGACG	ATCAATTTCATCTTCATCCTCCA	42.9	34.8	57.2	55.7	513-520 bp	1.0	41.1
rps19_p4	TGTTTCAATTCTTCGTCGACG	TCAATTTCATCTTCATCCTCCAT	42.9	34.8	57.2	55.7	512-519 bp	1.0	41.1
rps19_p5	GTTTCAATTCTTCGTCGACGT	TCAATTTCATCTTCATCCTCCAT	42.9	34.8	57.2	55.7	511-518 bp	1.0	41.1