Hotspot

rpoC2

Genome · 106593-110120 · 3528 bp

Back to Hesperocyparis

Pi 0.000425

MarkerSeek score 55.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: Genome
length_bp: 3528
variable_sites: 3
pi: 0.000425
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TTTCGTTTTTCCTACCCGCA	CCAGTGCATTTCTCCTGTCA	45.0	50.0	58.0	57.8	250 bp	1.0	78.8
rpoC2_p2	CAGAACATATACGAGCCCCG	CCAGTGCATTTCTCCTGTCA	55.0	50.0	57.9	57.8	299 bp	1.0	78.5
rpoC2_p3	TTTCGTTTTTCCTACCCGCA	CATTTCTCCTGTCAGGCCAG	45.0	55.0	58.0	58.3	244 bp	1.0	78.4
rpoC2_p4	CAGAACATATACGAGCCCCG	CATTTCTCCTGTCAGGCCAG	55.0	55.0	57.9	58.3	293 bp	1.0	78.0
rpoC2_p5	TTTCGTTTTTCCTACCCGCA	TTCTCCTGTCAGGCCAGAAT	45.0	50.0	58.0	58.3	241 bp	1.0	77.7