Hotspot

atpI-atpH

Genome · 111935-112497 · 563 bp

Back to Hesperocyparis

Pi 0.000888

MarkerSeek score 49.90

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: Genome
length_bp: 563
variable_sites: 1
pi: 0.000888
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	ACAAGTGGCATTCAAGCTCT	CCTACGGATAATCCAGCAGC	45.0	55.0	57.7	57.9	691-694 bp	1.0	77.5
atpI-atpH_p2	ACAAGTGGCATTCAAGCTCT	AACGGAAGCAGCAGAAATCA	45.0	45.0	57.7	58.1	668-671 bp	1.0	77.4
atpI-atpH_p3	ACAAGTGGCATTCAAGCTCT	ACGGAAGCAGCAGAAATCAA	45.0	45.0	57.7	58.1	667-670 bp	1.0	77.4
atpI-atpH_p4	ACAAGTGGCATTCAAGCTCT	GCCAGGTCCAATAGAAGCAA	45.0	50.0	57.7	57.9	713-716 bp	1.0	77.2
atpI-atpH_p5	ACAAGTGGCATTCAAGCTCT	TCCAGCAGCAATAACGGAAG	45.0	50.0	57.7	58.3	680-683 bp	1.0	76.1