Hotspot

psbM-petN

Genome · 98635-99239 · 605 bp

Back to Hesperocyparis

Pi 0.00331

MarkerSeek score 41.50

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 605
variable_sites: 4
pi: 0.00331
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	GCACAAACGAAGCCTCAAAG	TGGGCTGCTCTAATGGTAGT	50.0	50.0	58.2	58.1	707 bp	1.0	77.6
psbM-petN_p2	ATTTACGCACAAACGAAGCC	TGGGCTGCTCTAATGGTAGT	45.0	50.0	57.7	58.1	713 bp	1.0	76.9
psbM-petN_p3	TATTCATTGCAGTGCCCACT	TGGGCTGCTCTAATGGTAGT	45.0	50.0	57.5	58.1	748 bp	1.0	75.4
psbM-petN_p4	CGTCATTTACGCACAAACGA	TGGGCTGCTCTAATGGTAGT	45.0	50.0	57.4	58.1	717 bp	1.0	75.0
psbM-petN_p5	ACGAAGCCTCAAAGTGAACT	TGGGCTGCTCTAATGGTAGT	45.0	50.0	57.4	58.1	701 bp	1.0	74.5