Hotspot

trnS-GGA-ycf3

Genome · 84825-85519 · 695 bp

Back to Hesperocyparis

Pi 0.0024

MarkerSeek score 37.80

Primer available yes

Label trnS-GGA-ycf3

Feature Metadata

label_name: trnS-GGA-ycf3
feature_type: igs
region: Genome
length_bp: 695
variable_sites: 3
pi: 0.0024
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-ycf3_p1	CCGCTCAGCCATCTTTCC	GCCATCGGGATTATCTGCAA	61.1	50.0	58.2	58.1	796-807 bp	1.0	62.3
trnS-GGA-ycf3_p2	CCGCTCAGCCATCTTTCC	AAGCCATCGGGATTATCTGC	61.1	50.0	58.2	57.8	798-809 bp	1.0	61.6
trnS-GGA-ycf3_p3	CCGCTCAGCCATCTTTCC	CTTTTTCTCCCCTGAAGCCA	61.1	50.0	58.2	57.7	812-823 bp	1.0	60.8
trnS-GGA-ycf3_p4	CCGCTCAGCCATCTTTCC	TGCCTTTTTCTCCCCTGAAG	61.1	50.0	58.2	57.7	815-826 bp	1.0	60.8
trnS-GGA-ycf3_p5	CCGCTCAGCCATCTTTCC	CCTTTTTCTCCCCTGAAGCC	61.1	55.0	58.2	58.4	813-824 bp	1.0	59.5