Hotspot

ndhF-rpl32

Genome · 73143-73859 · 717 bp

Back to Hesperocyparis

Pi 0.00279

MarkerSeek score 51.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 717
variable_sites: 2
pi: 0.00279
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AACACTCTTGGTTGCTCCTG	TTTCTTGCGTCCGAATTTGC	50.0	45.0	58.0	58.0	910-920 bp	1.0	79.9
ndhF-rpl32_p2	AACACTCTTGGTTGCTCCTG	CTTGCGTCCGAATTTGCTTT	50.0	45.0	58.0	57.9	907-917 bp	1.0	79.9
ndhF-rpl32_p3	GAACACTCTTGGTTGCTCCT	TTTCTTGCGTCCGAATTTGC	50.0	45.0	57.7	58.0	911-921 bp	1.0	78.1
ndhF-rpl32_p4	GAACACTCTTGGTTGCTCCT	CTTGCGTCCGAATTTGCTTT	50.0	45.0	57.7	57.9	908-918 bp	1.0	78.0
ndhF-rpl32_p5	CTCTTGGTTGCTCCTGGAAA	TTTCTTGCGTCCGAATTTGC	50.0	45.0	57.7	58.0	906-916 bp	1.0	78.0