Hotspot

trnV-GAC-rrn16

Genome · 61027-61578 · 552 bp

Back to Hesperocyparis

Pi 0.00181
MarkerSeek score 59.40
Primer available yes
Label trnV-GAC-rrn16

Feature Metadata

label_name
trnV-GAC-rrn16
feature_type
igs
region
Genome
length_bp
552
variable_sites
2
pi
0.00181
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rrn16_p1 ACCTTGACGTGGTGAAAGTC GGGCAGGTTCTTACGCATTA 50.0 50.0 58.1 58.0 714-721 bp 1.0 79.8
trnV-GAC-rrn16_p2 CCTTGACGTGGTGAAAGTCA GGGCAGGTTCTTACGCATTA 50.0 50.0 58.1 58.0 713-720 bp 1.0 79.8
trnV-GAC-rrn16_p3 ACCTTGACGTGGTGAAAGTC TACGCATTACTCACCCGTTC 50.0 50.0 58.1 57.7 703-710 bp 1.0 77.8
trnV-GAC-rrn16_p4 CCTTGACGTGGTGAAAGTCA TACGCATTACTCACCCGTTC 50.0 50.0 58.1 57.7 702-709 bp 1.0 77.8
trnV-GAC-rrn16_p5 ACCTTGACGTGGTGAAAGTC CTTACGCATTACTCACCCGT 50.0 50.0 58.1 57.7 705-712 bp 1.0 77.7