Hotspot

rps12-trnV-GAC

Genome · 60094-60954 · 861 bp

Back to Hesperocyparis

Pi 0.00116
MarkerSeek score 59.70
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
Genome
length_bp
861
variable_sites
2
pi
0.00116
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 ATCGTACTCTGGCGACTTTG GACTTTCACCACGTCAAGGT 50.0 50.0 57.7 58.1 963-964 bp 1.0 77.6
rps12-trnV-GAC_p2 ATCGTACTCTGGCGACTTTG TGACTTTCACCACGTCAAGG 50.0 50.0 57.7 58.1 964-965 bp 1.0 77.6
rps12-trnV-GAC_p3 GCGTAAGGCAGAGTTTGGTT GACTTTCACCACGTCAAGGT 50.0 50.0 58.8 58.1 944-945 bp 1.0 73.7
rps12-trnV-GAC_p4 GCGTAAGGCAGAGTTTGGTT TGACTTTCACCACGTCAAGG 50.0 50.0 58.8 58.1 945-946 bp 1.0 73.7
rps12-trnV-GAC_p5 ATCGTACTCTGGCGACTTTG AGGTGACACTCTACCACTGA 50.0 50.0 57.7 57.3 947-948 bp 1.0 72.6