Hotspot

accD-rpl23

Genome · 12856-13915 · 1060 bp

Back to Hesperocyparis

Pi 0
MarkerSeek score 50.00
Primer available yes
Label accD-rpl23

Feature Metadata

label_name
accD-rpl23
feature_type
igs
region
Genome
length_bp
1060
variable_sites
0
pi
0
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-rpl23_p1 CATGGTTCCACGAGCTCTTT TTGATCCCGAATCGACATTT 50.0 40.0 58.2 55.1 1222-1227 bp 1.0 56.1
accD-rpl23_p2 CATGGTTCCACGAGCTCTTT TTTGATCCCGAATCGACATT 50.0 40.0 58.2 55.1 1223-1228 bp 1.0 56.1
accD-rpl23_p3 CATGGTTCCACGAGCTCTTT TTTGATCCCGAATCGACATTT 50.0 38.1 58.2 56.0 1223-1228 bp 1.0 54.5
accD-rpl23_p4 CATGGTTCCACGAGCTCTTT ATTTGATCCCGAATCGACAT 50.0 40.0 58.2 54.9 1224-1229 bp 1.0 54.2
accD-rpl23_p5 ATGGTTCCACGAGCTCTTTT TTGATCCCGAATCGACATTT 45.0 40.0 57.4 55.1 1221-1226 bp 1.0 53.0