primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
accD-rpl23_p1	accD-rpl23	1	CATGGTTCCACGAGCTCTTT	TTGATCCCGAATCGACATTT	20	20	50.0	40.0	58.2	55.1	12.8	3.4	3.040480	12856	13915	1222-1227	1.000000	56.1
accD-rpl23_p2	accD-rpl23	2	CATGGTTCCACGAGCTCTTT	TTTGATCCCGAATCGACATT	20	20	50.0	40.0	58.2	55.1	12.8	3.4	3.040480	12856	13915	1223-1228	1.000000	56.1
accD-rpl23_p3	accD-rpl23	3	CATGGTTCCACGAGCTCTTT	TTTGATCCCGAATCGACATTT	20	21	50.0	38.1	58.2	56.0	12.8	3.4	3.232014	12856	13915	1223-1228	1.000000	54.5
accD-rpl23_p4	accD-rpl23	4	CATGGTTCCACGAGCTCTTT	ATTTGATCCCGAATCGACAT	20	20	50.0	40.0	58.2	54.9	12.8	3.4	3.276656	12856	13915	1224-1229	1.000000	54.2
accD-rpl23_p5	accD-rpl23	5	ATGGTTCCACGAGCTCTTTT	TTGATCCCGAATCGACATTT	20	20	45.0	40.0	57.4	55.1	12.8	3.4	3.422616	12856	13915	1221-1226	1.000000	53.0
rps19_p1	rps19	1	TGTTTCAATTCTTCGTCGACG	TCAATTTCATCTTCATCCTCCA	21	22	42.9	36.4	57.2	55.1	31.3	0.0	6.628146	15760	16125	512-519	1.000000	41.1
rps19_p2	rps19	2	GTTTCAATTCTTCGTCGACGT	TCAATTTCATCTTCATCCTCCA	21	22	42.9	36.4	57.2	55.1	34.4	0.0	6.629895	15760	16125	511-518	1.000000	41.1
rps19_p3	rps19	3	TGTTTCAATTCTTCGTCGACG	ATCAATTTCATCTTCATCCTCCA	21	23	42.9	34.8	57.2	55.7	31.3	0.0	7.079869	15760	16125	513-520	1.000000	41.1
rps19_p4	rps19	4	TGTTTCAATTCTTCGTCGACG	TCAATTTCATCTTCATCCTCCAT	21	23	42.9	34.8	57.2	55.7	31.3	0.0	7.079869	15760	16125	512-519	1.000000	41.1
rps19_p5	rps19	5	GTTTCAATTCTTCGTCGACGT	TCAATTTCATCTTCATCCTCCAT	21	23	42.9	34.8	57.2	55.7	34.4	0.0	7.081618	15760	16125	511-518	1.000000	41.1
rps12-clpP_p1	rps12-clpP	1	GCATACTCCCTTCCGTTGAG	GCAGGTTGTTTTTCTAGCGC	20	20	55.0	50.0	58.1	58.3	0.0	0.0	0.372211	36519	37682	1326-1343	1.000000	78.8
rps12-clpP_p2	rps12-clpP	2	TTTCTTATCGGCTGTCTCGC	GCAGGTTGTTTTTCTAGCGC	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.390965	36519	37682	1274-1291	1.000000	78.7
rps12-clpP_p3	rps12-clpP	3	CTTATCGGCTGTCTCGCATT	GCAGGTTGTTTTTCTAGCGC	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.455444	36519	37682	1271-1288	1.000000	78.2
rps12-clpP_p4	rps12-clpP	4	TATCGGCTGTCTCGCATTTC	GCAGGTTGTTTTTCTAGCGC	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.460594	36519	37682	1269-1286	1.000000	78.2
rps12-clpP_p5	rps12-clpP	5	TTATCGGCTGTCTCGCATTT	GCAGGTTGTTTTTCTAGCGC	20	20	45.0	50.0	57.7	58.3	0.0	0.0	0.623064	36519	37682	1270-1287	1.000000	76.9
ycf1-trnL-CAA_p1	ycf1-trnL-CAA	1	CGGACCAATTGGCAATGTTG	CATTTTGAGTGTCGCGTGTC	20	20	50.0	50.0	58.3	58.1	24.8	5.8	0.350240	47070	47966	1033-1250	1.000000	77.5
ycf1-trnL-CAA_p2	ycf1-trnL-CAA	2	CGGACCAATTGGCAATGTTG	GACATTTTGAGTGTCGCGTG	20	20	50.0	50.0	58.3	58.1	24.8	5.8	0.350240	47070	47966	1035-1252	1.000000	77.5
ycf1-trnL-CAA_p3	ycf1-trnL-CAA	3	CGGACCAATTGGCAATGTTG	ATTTTGAGTGTCGCGTGTCT	20	20	50.0	45.0	58.3	58.1	24.8	5.8	0.421734	47070	47966	1032-1249	1.000000	77.0
ycf1-trnL-CAA_p4	ycf1-trnL-CAA	4	CGGACCAATTGGCAATGTTG	TTTGAGTGTCGCGTGTCTAC	20	20	50.0	50.0	58.3	58.2	24.8	5.8	0.525180	47070	47966	1030-1247	1.000000	76.1
ycf1-trnL-CAA_p5	ycf1-trnL-CAA	5	AAATACATTTGGGCCGGACC	CATTTTGAGTGTCGCGTGTC	20	20	50.0	50.0	58.5	58.1	23.7	5.8	0.581851	47070	47966	1047-1264	1.000000	75.7
ycf2_p1	ycf2	1	TACTCGGGTTCATAGCGAGA	AGAAATGCCGATCTTCGCTG	20	20	50.0	50.0	57.7	58.7	0.0	0.0	1.043021	50499	50618	261-261	1.000000	72.0
ycf2_p2	ycf2	2	TACTCGGGTTCATAGCGAGA	AGCAGAAATGCCGATCTTCG	20	20	50.0	50.0	57.7	58.7	0.0	0.0	1.043021	50499	50618	264-264	1.000000	72.0
ycf2_p3	ycf2	3	TACTCGGGTTCATAGCGAGA	CAGAAATGCCGATCTTCGCT	20	20	50.0	50.0	57.7	58.7	0.0	0.0	1.043021	50499	50618	262-262	1.000000	72.0
ycf2_p4	ycf2	4	GGGTTCATAGCGAGACAGAA	AGAAATGCCGATCTTCGCTG	20	20	50.0	50.0	57.0	58.7	0.0	0.0	1.667602	50499	50618	256-256	1.000000	67.0
ycf2_p5	ycf2	5	GGGTTCATAGCGAGACAGAA	CAGAAATGCCGATCTTCGCT	20	20	50.0	50.0	57.0	58.7	0.0	0.0	1.667602	50499	50618	257-257	1.000000	67.0
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	ATCGTACTCTGGCGACTTTG	GACTTTCACCACGTCAAGGT	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.330117	60094	60954	963-964	1.000000	77.6
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	ATCGTACTCTGGCGACTTTG	TGACTTTCACCACGTCAAGG	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.331478	60094	60954	964-965	1.000000	77.6
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	GCGTAAGGCAGAGTTTGGTT	GACTTTCACCACGTCAAGGT	20	20	50.0	50.0	58.8	58.1	0.0	0.0	0.814811	60094	60954	944-945	1.000000	73.7
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	GCGTAAGGCAGAGTTTGGTT	TGACTTTCACCACGTCAAGG	20	20	50.0	50.0	58.8	58.1	0.0	0.0	0.816172	60094	60954	945-946	1.000000	73.7
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	ATCGTACTCTGGCGACTTTG	AGGTGACACTCTACCACTGA	20	20	50.0	50.0	57.7	57.3	0.0	5.4	0.957125	60094	60954	947-948	1.000000	72.6
trnV-GAC-rrn16_p1	trnV-GAC-rrn16	1	ACCTTGACGTGGTGAAAGTC	GGGCAGGTTCTTACGCATTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.083215	61027	61578	714-721	1.000000	79.8
trnV-GAC-rrn16_p2	trnV-GAC-rrn16	2	CCTTGACGTGGTGAAAGTCA	GGGCAGGTTCTTACGCATTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.084576	61027	61578	713-720	1.000000	79.8
trnV-GAC-rrn16_p3	trnV-GAC-rrn16	3	ACCTTGACGTGGTGAAAGTC	TACGCATTACTCACCCGTTC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.333235	61027	61578	703-710	1.000000	77.8
trnV-GAC-rrn16_p4	trnV-GAC-rrn16	4	CCTTGACGTGGTGAAAGTCA	TACGCATTACTCACCCGTTC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.334596	61027	61578	702-709	1.000000	77.8
trnV-GAC-rrn16_p5	trnV-GAC-rrn16	5	ACCTTGACGTGGTGAAAGTC	CTTACGCATTACTCACCCGT	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.339495	61027	61578	705-712	1.000000	77.7
rrn16-trnI-GAU_p1	rrn16-trnI-GAU	1	AGTGACTGGAGTGAAGTCGT	ACCACTGAGCTAATAGCCCA	20	20	50.0	50.0	58.3	58.1	0.0	8.9	0.426799	63065	64030	1089-1101	1.000000	77.2
rrn16-trnI-GAU_p2	rrn16-trnI-GAU	2	AAGTCGTAACAAGGTAGCCG	ACCACTGAGCTAATAGCCCA	20	20	50.0	50.0	57.6	58.1	0.0	8.9	0.479364	63065	64030	1076-1088	1.000000	76.8
rrn16-trnI-GAU_p3	rrn16-trnI-GAU	3	AACAAGGTAGCCGTACTGGA	ACCACTGAGCTAATAGCCCA	20	20	50.0	50.0	58.4	58.1	7.8	8.9	0.484277	63065	64030	1069-1081	1.000000	76.7
rrn16-trnI-GAU_p4	rrn16-trnI-GAU	4	GGCTAGTGACTGGAGTGAAG	ACCACTGAGCTAATAGCCCA	20	20	55.0	50.0	57.3	58.1	0.0	8.9	0.784251	63065	64030	1093-1105	1.000000	74.3
rrn16-trnI-GAU_p5	rrn16-trnI-GAU	5	TGCCATGCTCGAAGTCATTA	ACCACTGAGCTAATAGCCCA	20	20	45.0	50.0	57.3	58.1	0.0	8.9	0.809978	63065	64030	1146-1158	1.000000	74.1
ndhF-rpl32_p1	ndhF-rpl32	1	AACACTCTTGGTTGCTCCTG	TTTCTTGCGTCCGAATTTGC	20	20	50.0	45.0	58.0	58.0	0.8	0.0	0.072016	73143	73859	910-920	1.000000	79.9
ndhF-rpl32_p2	ndhF-rpl32	2	AACACTCTTGGTTGCTCCTG	CTTGCGTCCGAATTTGCTTT	20	20	50.0	45.0	58.0	57.9	0.8	0.0	0.077605	73143	73859	907-917	1.000000	79.9
ndhF-rpl32_p3	ndhF-rpl32	3	GAACACTCTTGGTTGCTCCT	TTTCTTGCGTCCGAATTTGC	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.307311	73143	73859	911-921	1.000000	78.1
ndhF-rpl32_p4	ndhF-rpl32	4	GAACACTCTTGGTTGCTCCT	CTTGCGTCCGAATTTGCTTT	20	20	50.0	45.0	57.7	57.9	0.0	0.0	0.312900	73143	73859	908-918	1.000000	78.0
ndhF-rpl32_p5	ndhF-rpl32	5	CTCTTGGTTGCTCCTGGAAA	TTTCTTGCGTCCGAATTTGC	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.321620	73143	73859	906-916	1.000000	78.0
trnS-GGA-ycf3_p1	trnS-GGA-ycf3	1	CCGCTCAGCCATCTTTCC	GCCATCGGGATTATCTGCAA	18	20	61.1	50.0	58.2	58.1	0.0	0.0	2.284593	84825	85519	796-807	1.000000	62.3
trnS-GGA-ycf3_p2	trnS-GGA-ycf3	2	CCGCTCAGCCATCTTTCC	AAGCCATCGGGATTATCTGC	18	20	61.1	50.0	58.2	57.8	0.0	0.0	2.371940	84825	85519	798-809	1.000000	61.6
trnS-GGA-ycf3_p3	trnS-GGA-ycf3	3	CCGCTCAGCCATCTTTCC	CTTTTTCTCCCCTGAAGCCA	18	20	61.1	50.0	58.2	57.7	0.0	0.0	2.473641	84825	85519	812-823	1.000000	60.8
trnS-GGA-ycf3_p4	trnS-GGA-ycf3	4	CCGCTCAGCCATCTTTCC	TGCCTTTTTCTCCCCTGAAG	18	20	61.1	50.0	58.2	57.7	0.0	0.0	2.473641	84825	85519	815-826	1.000000	60.8
trnS-GGA-ycf3_p5	trnS-GGA-ycf3	5	CCGCTCAGCCATCTTTCC	CCTTTTTCTCCCCTGAAGCC	18	20	61.1	55.0	58.2	58.4	0.0	0.0	2.630486	84825	85519	813-824	1.000000	59.5
ycf3_p1	ycf3	1	TTATGCTATTGTGCGTTGGC	AGCTAATTCGTTCGAATAGCA	20	21	45.0	38.1	57.2	55.5	0.0	28.0	4.320426	85520	87436	2062-2063	1.000000	45.6
ycf3_p2	ycf3	2	TTATGCTATTGTGCGTTGGC	GAAGCTAATTCGTTCGAATAGCA	20	23	45.0	39.1	57.2	57.4	0.0	28.0	4.479312	85520	87436	2064-2065	1.000000	44.3
ycf3_p3	ycf3	3	TTATGCTATTGTGCGTTGGC	AAGCTAATTCGTTCGAATAGCA	20	22	45.0	36.4	57.2	56.3	0.0	28.0	4.572321	85520	87436	2063-2064	1.000000	43.6
ycf3_p4	ycf3	4	TTATGCTATTGTGCGTTGGC	AGAGAAGCTAATTCGTTCGA	20	20	45.0	40.0	57.2	54.3	0.0	0.0	4.583618	85520	87436	2067-2068	1.000000	43.5
ycf3_p5	ycf3	5	TTATGCTATTGTGCGTTGGC	AGAAGCTAATTCGTTCGAATAGC	20	23	45.0	39.1	57.2	57.1	0.0	24.1	4.734018	85520	87436	2065-2066	1.000000	42.3
psbM-petN_p1	psbM-petN	1	GCACAAACGAAGCCTCAAAG	TGGGCTGCTCTAATGGTAGT	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.361642	98635	99239	707-707	1.000000	77.6
psbM-petN_p2	psbM-petN	2	ATTTACGCACAAACGAAGCC	TGGGCTGCTCTAATGGTAGT	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.445935	98635	99239	713-713	1.000000	76.9
psbM-petN_p3	psbM-petN	3	TATTCATTGCAGTGCCCACT	TGGGCTGCTCTAATGGTAGT	20	20	45.0	50.0	57.5	58.1	13.7	0.0	0.633117	98635	99239	748-748	1.000000	75.4
psbM-petN_p4	psbM-petN	4	CGTCATTTACGCACAAACGA	TGGGCTGCTCTAATGGTAGT	20	20	45.0	50.0	57.4	58.1	0.0	0.0	0.684095	98635	99239	717-717	1.000000	75.0
psbM-petN_p5	psbM-petN	5	ACGAAGCCTCAAAGTGAACT	TGGGCTGCTCTAATGGTAGT	20	20	45.0	50.0	57.4	58.1	0.0	0.0	0.742489	98635	99239	701-701	1.000000	74.5
rpoC2_p1	rpoC2	1	TTTCGTTTTTCCTACCCGCA	CCAGTGCATTTCTCCTGTCA	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.225041	107780	107929	250-250	1.000000	78.8
rpoC2_p2	rpoC2	2	CAGAACATATACGAGCCCCG	CCAGTGCATTTCTCCTGTCA	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.255586	107780	107929	299-299	1.000000	78.5
rpoC2_p3	rpoC2	3	TTTCGTTTTTCCTACCCGCA	CATTTCTCCTGTCAGGCCAG	20	20	45.0	55.0	58.0	58.3	0.0	14.6	0.287738	107780	107929	244-244	1.000000	78.4
rpoC2_p4	rpoC2	4	CAGAACATATACGAGCCCCG	CATTTCTCCTGTCAGGCCAG	20	20	55.0	55.0	57.9	58.3	0.0	14.6	0.318283	107780	107929	293-293	1.000000	78.0
rpoC2_p5	rpoC2	5	TTTCGTTTTTCCTACCCGCA	TTCTCCTGTCAGGCCAGAAT	20	20	45.0	50.0	58.0	58.3	0.0	14.6	0.373984	107780	107929	241-241	1.000000	77.7
atpI-atpH_p1	atpI-atpH	1	ACAAGTGGCATTCAAGCTCT	CCTACGGATAATCCAGCAGC	20	20	45.0	55.0	57.7	57.9	0.0	0.0	0.369200	111935	112497	691-694	1.000000	77.5
atpI-atpH_p2	atpI-atpH	2	ACAAGTGGCATTCAAGCTCT	AACGGAAGCAGCAGAAATCA	20	20	45.0	45.0	57.7	58.1	0.0	0.0	0.386926	111935	112497	668-671	1.000000	77.4
atpI-atpH_p3	atpI-atpH	3	ACAAGTGGCATTCAAGCTCT	ACGGAAGCAGCAGAAATCAA	20	20	45.0	45.0	57.7	58.1	0.0	0.0	0.386926	111935	112497	667-670	1.000000	77.4
atpI-atpH_p4	atpI-atpH	4	ACAAGTGGCATTCAAGCTCT	GCCAGGTCCAATAGAAGCAA	20	20	45.0	50.0	57.7	57.9	0.0	0.0	0.411916	111935	112497	713-716	1.000000	77.2
atpI-atpH_p5	atpI-atpH	5	ACAAGTGGCATTCAAGCTCT	TCCAGCAGCAATAACGGAAG	20	20	45.0	50.0	57.7	58.3	0.0	0.0	0.545307	111935	112497	680-683	1.000000	76.1
psbI-psbK_p1	psbI-psbK	1	TCTTTACGCCCAGGATTTCG	CAAGCTGCCGTAAGTTTTCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.068070	118081	118685	735-736	1.000000	79.7
psbI-psbK_p2	psbI-psbK	2	TCTTTACGCCCAGGATTTCG	AGCTGCCGTAAGTTTTCGAT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.113304	118081	118685	733-734	1.000000	79.3
psbI-psbK_p3	psbI-psbK	3	TCTTTACGCCCAGGATTTCG	TTTGCCAAATTGCCTGAAGC	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.136951	118081	118685	831-832	1.000000	79.1
psbI-psbK_p4	psbI-psbK	4	TCTTTACGCCCAGGATTTCG	AAGCTGCCGTAAGTTTTCGA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.144844	118081	118685	734-735	1.000000	79.1
psbI-psbK_p5	psbI-psbK	5	CTTTACGCCCAGGATTTCGT	CAAGCTGCCGTAAGTTTTCG	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.315366	118081	118685	734-735	1.000000	77.7
chlB-trnK-UUU_p1	chlB-trnK-UUU	1	TTGCACGACAAAAGGGGATT	CCGAGTACTCTGCCATTGAG	20	20	45.0	55.0	58.3	57.8	0.0	20.3	0.517401	120947	122050	1204-1208	1.000000	76.3
chlB-trnK-UUU_p2	chlB-trnK-UUU	2	TTTGCACGACAAAAGGGGAT	CCGAGTACTCTGCCATTGAG	20	20	45.0	55.0	58.3	57.8	6.0	20.3	0.517401	120947	122050	1205-1209	1.000000	76.3
chlB-trnK-UUU_p3	chlB-trnK-UUU	3	TTGCACGACAAAAGGGGATT	TGCCATTGAGTTAGCAACCC	20	20	45.0	50.0	58.3	58.5	0.0	0.0	0.756770	120947	122050	1194-1198	1.000000	74.3
chlB-trnK-UUU_p4	chlB-trnK-UUU	4	TTTGCACGACAAAAGGGGAT	TGCCATTGAGTTAGCAACCC	20	20	45.0	50.0	58.3	58.5	6.0	0.0	0.756770	120947	122050	1195-1199	1.000000	74.3
chlB-trnK-UUU_p5	chlB-trnK-UUU	5	TCCTCGTTTTGCCAGAGAAA	CCGAGTACTCTGCCATTGAG	20	20	45.0	55.0	57.4	57.8	0.0	20.3	0.847067	120947	122050	1248-1252	1.000000	73.6