Hotspot

rps15-trnN-GUU

SSC · 123318-129192 · 5875 bp

Back to Hesperis

Pi 0.0136

MarkerSeek score 56.10

Primer available yes

Label rps15-trnN-GUU

Feature Metadata

label_name: rps15-trnN-GUU
feature_type: igs
region: SSC
length_bp: 5875
variable_sites: 130
pi: 0.0136
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN-GUU_p1	GTTTTACGCCTTTGGGAACG	GGATCTCTCGCGGAAGAATC	50.0	55.0	58.0	57.9	604-605 bp	1.0	82.8
rps15-trnN-GUU_p2	AATAGGGCTTTGTTTGGGCA	GGATCTCTCGCGGAAGAATC	45.0	55.0	58.0	57.9	1236-1251 bp	1.0	82.4
rps15-trnN-GUU_p3	AATAGGGCTTTGTTTGGGCA	TGCCAAAGTGATGGAAACGA	45.0	45.0	58.0	58.0	1543-1558 bp	1.0	82.3
rps15-trnN-GUU_p4	AATAGGGCTTTGTTTGGGCA	TGATCCATTTTTGCGTGGGA	45.0	45.0	58.0	58.1	1253-1268 bp	1.0	82.3
rps15-trnN-GUU_p5	GTTTTACGCCTTTGGGAACG	TGCCAAAGTGATGGAAACGA	50.0	45.0	58.0	58.0	911-912 bp	1.0	82.2