Hotspot

psbE-petL

LSC · 64058-65411 · 1354 bp

Back to Hesperis

Pi 0.0161
MarkerSeek score 61.80
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1354
variable_sites
36
pi
0.0161
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC AGCTGCTAGTAGAAAACCGA 50.0 45.0 57.8 56.3 1412-1427 bp 1.0 68.7
psbE-petL_p2 GTTCTCCTGTGCTTCCAGAC AGCTGCTAGTAGAAAACCGA 55.0 45.0 58.2 56.3 1407-1422 bp 1.0 68.3
psbE-petL_p3 TCTCCTGTGCTTCCAGACAT AGCTGCTAGTAGAAAACCGA 50.0 45.0 58.4 56.3 1405-1420 bp 1.0 67.1
psbE-petL_p4 AGAACGTTCTCCTGTGCTTC AAGCTGCTAGTAGAAAACCGA 50.0 42.9 57.8 57.0 1413-1428 bp 1.0 66.7
psbE-petL_p5 AGAACGTTCTCCTGTGCTTC AGCTGCTAGTAGAAAACCGAA 50.0 42.9 57.8 57.0 1412-1427 bp 1.0 66.7