Hotspot

trnM-CAU-atpE

LSC · 51875-52008 · 134 bp

Back to Hesperis

Pi 0.0188

MarkerSeek score 61.90

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 134
variable_sites: 5
pi: 0.0188
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGCAGGAGTCATTGGTTCAA	AGTCGAGGCTCTCAATACGA	50.0	50.0	58.1	58.0	188-194 bp	1.0	84.8
trnM-CAU-atpE_p2	GGCAGGAGTCATTGGTTCAA	ACGAGTCGAGGCTCTCAATA	50.0	50.0	58.1	58.0	191-197 bp	1.0	84.7
trnM-CAU-atpE_p3	TTGCTTTCATACGGCAGGAG	AGTCGAGGCTCTCAATACGA	50.0	50.0	58.3	58.0	200-206 bp	1.0	83.1
trnM-CAU-atpE_p4	TTGCTTTCATACGGCAGGAG	ACGAGTCGAGGCTCTCAATA	50.0	50.0	58.3	58.0	203-209 bp	1.0	83.0
trnM-CAU-atpE_p5	CATACGGCAGGAGTCATTGG	AGTCGAGGCTCTCAATACGA	55.0	50.0	58.4	58.0	193-199 bp	1.0	82.1