Hotspot

trnT-GGU-psbD

LSC · 31548-32770 · 1223 bp

Back to Hesperis

Pi 0.0117

MarkerSeek score 60.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1223
variable_sites: 24
pi: 0.0117
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	TCCCTCCGTAACCAGTCATC	55.0	55.0	58.1	58.5	1242-1358 bp	1.0	80.8
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	TCCCTCCGTAACCAGTCATC	50.0	55.0	58.1	58.5	1245-1361 bp	1.0	80.6
trnT-GGU-psbD_p3	CCAAGACGGCCCTTTTAACT	TCCCTCCGTAACCAGTCATC	50.0	55.0	58.1	58.5	1264-1380 bp	1.0	80.6
trnT-GGU-psbD_p4	ACGGCCCTTTTAACTCAGTG	TCCCTCCGTAACCAGTCATC	50.0	55.0	58.1	58.5	1259-1375 bp	1.0	80.4
trnT-GGU-psbD_p5	GACGGCCCTTTTAACTCAGT	TCCCTCCGTAACCAGTCATC	50.0	55.0	57.8	58.5	1260-1376 bp	1.0	79.9