Hotspot

trnS-GCU-trnG

LSC · 7917-8665 · 749 bp

Back to Hesperis

Pi 0.0437

MarkerSeek score 85.00

Primer available yes

Label trnS-GCU-trnG

Feature Metadata

label_name: trnS-GCU-trnG
feature_type: igs
region: LSC
length_bp: 749
variable_sites: 45
pi: 0.0437
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG_p1	CCAACGCTTTAGTCCACTCA	TGAATCAAACCGAGAGACCC	50.0	50.0	57.8	57.2	657-859 bp	1.0	81.2
trnS-GCU-trnG_p2	AACGCTTTAGTCCACTCAGC	TGAATCAAACCGAGAGACCC	50.0	50.0	58.2	57.2	655-857 bp	1.0	80.9
trnS-GCU-trnG_p3	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAGAGACCC	50.0	50.0	57.6	57.2	652-854 bp	1.0	79.3
trnS-GCU-trnG_p4	TCCAACGCTTTAGTCCACTC	TGAATCAAACCGAGAGACCC	50.0	50.0	57.5	57.2	658-860 bp	1.0	78.9
trnS-GCU-trnG_p5	CCAACGCTTTAGTCCACTCA	GAATCAAACCGAGAGACCCT	50.0	50.0	57.8	56.9	656-858 bp	1.0	78.8