Hotspot

trnK-UUU-rps16

LSC · 4339-5040 · 702 bp

Back to Hesperis

Pi 0.0283
MarkerSeek score 64.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
702
variable_sites
25
pi
0.0283
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.88
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG CCCAATGAGCCGTTTATCGA 55.0 50.0 58.0 58.1 977-1066 bp 1.0 86.1
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG TCCCAATGAGCCGTTTATCG 55.0 50.0 58.0 58.1 978-1067 bp 1.0 86.1
trnK-UUU-rps16_p3 TCAGGATCAGTCGTGGTCTT CCCAATGAGCCGTTTATCGA 50.0 50.0 58.1 58.1 973-1062 bp 1.0 85.7
trnK-UUU-rps16_p4 TCAGGATCAGTCGTGGTCTT TCCCAATGAGCCGTTTATCG 50.0 50.0 58.1 58.1 974-1063 bp 1.0 85.7
trnK-UUU-rps16_p5 TTCAGGATCAGTCGTGGTCT CCCAATGAGCCGTTTATCGA 50.0 50.0 58.1 58.1 974-1063 bp 1.0 85.7