Hotspot

trnH-GUG-psbA

LSC · 77-387 · 311 bp

Back to Hesperis

Pi 0.155

MarkerSeek score 74.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 311
variable_sites: 66
pi: 0.155
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	370-467 bp	1.0	90.7
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	GCGCTAACCTTGGTATGGAA	50.0	50.0	58.2	58.0	371-468 bp	1.0	90.5
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	320-417 bp	1.0	90.4
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	323-420 bp	1.0	90.3
trnH-GUG-psbA_p5	CTGCCTTAATCCACTTGGCT	GGTGTAGCTGGTGTATTCGG	50.0	55.0	57.9	58.1	737-834 bp	1.0	89.7