Hotspot

psbA

LSC · 85-1146 · 1062 bp

Back to Hesperaloe

Pi 0.00471

MarkerSeek score 50.40

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 5
pi: 0.00471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TTGGGTATGGCTCCTTCAAC	GTGCTTGGGAGTCCTTGAAA	50.0	50.0	57.5	58.0	1152 bp	1.0	76.1
psbA_p2	GGTATGGCTCCTTCAACGAT	GTGCTTGGGAGTCCTTGAAA	50.0	50.0	57.4	58.0	1149 bp	1.0	75.2
psbA_p3	TGGCTCCTTCAACGATTCTT	GTGCTTGGGAGTCCTTGAAA	45.0	50.0	57.1	58.0	1145 bp	1.0	73.3
psbA_p4	TTGGGTATGGCTCCTTCAAC	TAGTACGTGTGCTTGGGAGT	50.0	50.0	57.5	58.4	1160 bp	1.0	73.3
psbA_p5	TTGGGTATGGCTCCTTCAAC	GTTAGTACGTGTGCTTGGGA	50.0	50.0	57.5	57.6	1162 bp	1.0	72.7