Hotspot

trnN-GUU-trnR-ACG

IRa · 132397-133020 · 624 bp

Back to Hesperaloe

Pi 0.00481

MarkerSeek score 61.50

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 624
variable_sites: 3
pi: 0.00481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	765 bp	1.0	78.6
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCTGTGGAGACG	55.0	55.0	58.0	57.7	802 bp	1.0	78.0
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	716 bp	1.0	77.4
trnN-GUU-trnR-ACG_p4	CCGTTGTTCCTCAGTAGCTC	GGGAACAGATCCTGTGGAGA	55.0	55.0	58.0	58.4	804 bp	1.0	76.9
trnN-GUU-trnR-ACG_p5	TGGTCGTAGGTTCGAATCCT	GAACAGATCCTGTGGAGACG	50.0	55.0	58.2	57.7	753 bp	1.0	76.8