Hotspot

ndhG-ndhI

SSC · 121453-121813 · 361 bp

Back to Hesperaloe

Pi 0.00831

MarkerSeek score 51.30

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 361
variable_sites: 3
pi: 0.00831
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCGTGTATTGGTCCAGGTAA	GTCGGTTGCCAATGTCAGTA	45.0	50.0	55.9	58.2	503-505 bp	1.0	62.0
ndhG-ndhI_p2	TCGTGTATTGGTCCAGGTAAA	GTCGGTTGCCAATGTCAGTA	42.9	50.0	56.7	58.2	503-505 bp	1.0	60.2
ndhG-ndhI_p3	ATCGTGTATTGGTCCAGGTA	GTCGGTTGCCAATGTCAGTA	45.0	50.0	55.6	58.2	504-506 bp	1.0	60.1
ndhG-ndhI_p4	TATCGTGTATTGGTCCAGGT	GTCGGTTGCCAATGTCAGTA	45.0	50.0	55.6	58.2	505-507 bp	1.0	60.1
ndhG-ndhI_p5	TCGTGTATTGGTCCAGGTAA	TCGGTTGCCAATGTCAGTAA	45.0	45.0	55.9	57.4	502-504 bp	1.0	59.1